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Nucleotide sequences of primers that used in PCR for detection of E. coli pathotypes
| Target gene | Primer | Sequence(5'-3') | Size (bp) | Reference |
|---|---|---|---|---|
| stx1 | Stx1F | 5׳ GAAGAGTCCGTGGGATTACG | 130 | (12) |
| Stx1R | 5׳ AGCGATGCAGCTATTAATA | |||
| stx2 | Stx2F | 5׳ GGATGCATCTCTGGTCATTG | 478 | (13) |
| Stx2R | 5׳ CTTCGGTATCCTATTCCCGG | |||
| hly | HlyF | 5׳ AGCTGCAAGTGCGGGTCTG | 569 | (14) |
| HlyR | 5׳ TACGGGTTATGCCTGCAAGTTCAC | |||
| eae | EaeF | 5 ׳TCAATGCAGTTCCGTTATCAGTT | 482 | (15) |
| EaeR | 5׳ GTAAAGTCCGTTACCCCAACCTG | |||
| bfp | BfpF | 5׳ CACCGTTACCGCAGGTGTGA | 450 | (16) |
