Fig. 2.

PKA activation increases extrasynaptic α4 subunit abundance and function. Cortical neurons were exposed to vehicle or Sp-cAMP (50 μM) for 1 hour followed by either subsynaptic fractionation and western blot analysis or whole-cell patch clamp recording. Exposure to Sp-cAMP for 1 hour caused an increase in (A) extrasynaptic α4 and (B) δ subunit abundance as well as (C) tonic current blocked by bicuculline (20 μM) or (D) THIP-evoked (1 μM) current. *P < 0.05, **P < 0.01, Student’s t test, n = 7–13.