Fig. 5.

Andro reduces the adherence of monocytes/macrophages to ECs. (A) Fluorescently labeled RAW264.7 cells, pretreated with Andro (15 μM) or DMSO, were incubated with TNFα-treated (10 ng/ml) ECs. After washing vigorously, adhered RAW264.7 cells were imaged (upper panel) and quantified (lower panel). Data are expressed as the mean number of adhered cells/field ± S.E.M. (n = 4; *P < 0.05, one-way analysis of variance). (B–E) RAW264.7 cells were treated with or without Andro (15 μM) or solvent for the indicated time. mRNA expression of α4 (B), β1 (C), αL (D), and β2 (E) integrins was analyzed by quantitative real-time PCR. All values represent the mean ± S.E.M. (n = 4–6; *P < 0.05, one-way analysis of variance). N.S., not significant.