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. 2016 Jan 6;6:1192. doi: 10.3389/fpls.2015.01192

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Copyright © 2016 Nagaraj, Senthil-Kumar, Ramu, Wang and Mysore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing of NbRPL12 and NbRPL19 genes in N. benthamiana delayed development of HR in nonhost pathogen inoculated leaves. Using TRV-based VIGS, N. benthamiana plants were silenced for NbRPL12 or NbRPL19. Abaxial side of silenced and control (TRV2::GFP) leaves were inoculated (OD₆₀₀ = 0.001) using needleless syringe with nonhost pathogen P. syringae pv. tomato T1 (solid circles). The delay in HR symptoms were photographed at different days post inoculation. Similar response was observed in three individual experiments that included five biological replicates.