Figure 6.

Double-silenced plants showed delayed HR and enhanced bacterial multiplication upon inoculation with nonhost pathogens. The silenced leaves were inoculated using a needless syringe with nonhost pathogens P. syringae pv. tomato T1 (A, OD₆₀₀ = 0.001) and X. campestris pv. vesicatoria (B, OD₆₀₀ = 0.01). The delay in HR symptoms was photographed at the indicated time points post inoculation. Silenced leaves were dip inoculated with P. syringae pv. tomato T1 (OD600 = 0.01 for 5 min). The growth response of pathogen was quantified at 3 and 5 days post inoculation (DPI) (C). The error bars represent standard deviation from three replications. Different letters above the bar indicate a significant difference from Two-way ANOVA at p < 0.05 with Tukey's HSD means separation test (α = 0.05). See Supplementary Table S2 for details about statistics.