Table 2. Antibody details and conditions used for Western blotting and immunohistochemistry.
| Antibody | Supplier | Catalog no. | Application | Dilution |
|---|---|---|---|---|
| β-actin | Novus | NB600-501 | WB | 1:5000 |
| Lamin B1 | Santa cruz | sc-6216 | WB | 1:1000 |
| PPARγ | Cell Signaling | #2435 | WB/IHC | 1:1000/1:400 |
| t-ERK | Cell Signaling | #9102 | WB | 1:1000 |
| p-ERK | Cell Signaling | #9101 | WB | 1:1000 |
| t-AMPK | Cell Signaling | #2532 | WB | 1:1000 |
| p-AMPK | Cell Signaling | #2535 | WB | 1:1000 |
| t-Akt | Cell Signaling | #9272 | WB | 1:1000 |
| p-Akt | Cell Signaling | #4058 | WB | 1:1000 |
| t-CREB | Cell Signaling | #9197 | WB | 1:1000 |
| p-CREB | Cell Signaling | #9198 | WB | 1:1000 |
| FAS | Cell Signaling | #3180 | IHC | 1:100 |
| ACC | Cell Signaling | #3676 | IHC | 1:200 |
| Ang II | phoenix pharmaceuticals | H-002-12 | IHC | 1:2000 |
| p-p47 | Assay biotech | A1171 | IHC | 1:2500 |
| 4HNE | Alpha diagnostic | HNE11-S | IHC | 1:2500 |
| GLUT4 | Santa cruz | sc-7938 | IHC | 1:200 |
| p-IRS1 | Santa cruz | sc-17200 | IHC | 1:200 |
| p-Akt | Epitomics | #2118-S | IHC | 1:100 |
Antibodies were purchased from Cell signaling (Beverley, MA), Novus biological (Littleton, CO), Santa cruz (Santa Cruz Biotechnology, Inc., CA), phoenix pharmaceuticals (Burlingame, CA), Assay biotech (Sunnyvale, CA), Alpha diagnostic (San Antonio, TX), Epitomics (Burlingame, CA). WB: western blotting; IHC: immunohistochemistry.