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. 2016 Jan 6;6:18633. doi: 10.1038/srep18633

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Copyright © 2016, Macmillan Publishers Limited

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Pregnant CBA/J ×DBA/2 females or CBA non pregnant mice were sacrificed at 8.5 day of gestation and implantation sites were obtained. (A) VIP and (B) VPAC receptors were evaluated by RT-PCR as described in Mat erial and Methods (C) Implantation site explants isolated from CBAxDBA or CBAxBALB/c matings were cultured in the absence/presence of VIP (100 nM) during 24 h and IL-17 and RORγt were evaluated by RT-PCR. Bands were semi-quantified with ImageJ® and intensity expressed in arbitrary units (AU) relative to GAPDH. Values represent mean ± S.E.M of at least 3 experiments (Mann Whitney test *p < 0.05).