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. 2016 Jan 6;6:18633. doi: 10.1038/srep18633

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Copyright © 2016, Macmillan Publishers Limited

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CBA/J × DBA/2 pregnant mice were treated in vivo with 2 nmol/mouse VIP or PBS i.p. at day 6.5 and the implantation sites were obtained at day 8.5. (A) Foxp3 and RORγt expression and (B) TGF-β and PPARγ expression were evaluated by RT-PCR. Bands were semi-quantified with ImageJ® and intensity expressed in arbitrary units (AU) relative to GAPDH. Values represent mean ± S.E.M of at least 3 experiments (Mann Whitney test *p < 0.05).