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. 2016 Jan 6;6:18633. doi: 10.1038/srep18633

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Copyright © 2016, Macmillan Publishers Limited

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Peritoneal macrophages from CBA/J × DBA/2 females were recovered at 8.5 day of gestation and cultured with syngeneic thymocytes induced to apoptosis in the absence or presence of VIP (10 nM). After, 30, 60, 90 and 120 min cells were stained with Hematoxilin–Eosin and the (A) % of efferocytosis and (B) the phagocytic index were analyzed by microscopy. (C) Representative images of macrophages recovered from pregnant CBA/J treated with PBS or VIP after apoptotic thymocytes engulfment. (D) Maternal peritoneal macrophages were recovered at day 8.5 after in vivo treatment with VIP or PBS and cultured with with latex-beads-FITC conjugated. After 90 min the percentage of efferocytosis was quantified by FACS analysis. (E) Representative dot plot profile previous and after the efferocytosis assay in absence or presence of VIP.