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. 2016 Jan 6;6:18633. doi: 10.1038/srep18633

Figure 5. VIP treatment modulates macrophage cytokine production After the efferocytosis assay, macrophages from pregnant CBA/J mice treated with PBS or VIP (2 nmol/mouse) were washed and remained in culture for additional 24 h, then supernatants were collected and (A) IL-12 and (B) TNF-α were quantified by ELISA.

Figure 5

(C) Cells were recovered and the frequency of F4/80 + IL-10+ analyzed by FACS. The left pannel shows representative dot plot with the frequency of of F4/80 + IL-10 from famele treated in vivo with PBS or VIP, previous and after the efferocytosis assay.