Table 1. Primers used in RT-PCR assays.
| Primers | Forward | Reverse |
|---|---|---|
| GAPDH | TGATGACATCAAGAAGGTGGTGAAG | TCCTTGGAGGCCATGTAGGCCAT |
| VIP | TTCACCAGCGATTACAGCAG | TCACAGCCATTTGCTTTCTG |
| VPAC1 | GTGAAGACCGGCTACACCAT | TGAAGAGGGCCATATCCTTG |
| VPAC2 | GTGAAGACCGGCTACACCAT | TGAAGAGGGCCATATCCTTG |
| PPARγ | ATC TAC ACG ATG CTG GC | GGA TGT CCT CGA TGG |
| TGF-β | GACTCTCCACCTGCAAGACCA | TTGGGGGACTGGCGAGCCTT |
| Foxp3 | GGCCCTTCTCCAGGACAGA | GCTGATCATGGCTGGGTTGT |
| IL-17 | CTCCAGAAGGCCCTCAGACTAC | AGCTTTCCCCTCCGCATTGACACAG |
| RORγt | CACGGCCCTGGTTCTCAT | CAGATGTTCCACTCTCCTCTTCTCT |
Oligonucleotide primers were designed using the online tool Primer3® (Whitehead Institute for Biomedical Research).