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. 2016 Jan 6;6:18358. doi: 10.1038/srep18358

Figure 5. Expression profiling of OsDJ-1 genes under different developmental stages, various tissues and multiple abiotic and biotic stresses.

Figure 5

Expression was analyzed at nine distinct developmental stages (a) and sixteen tissues (b) of rice, as shown at the X-axis of the diagram. The mean signal intensity values from Affymetrix 51 K array were collected from genevestigator and plotted in the diagram. Color bar at the top of the figure represents the gene name. Expression of OsDJ-1 genes was further analyzed under different stress conditions by qRT-PCR. Bar graphs (c–h) depict fold change in expression of OsDJ-1 genes under different stress conditions – salt, cold, drought, heat, H2O2, MG, As, Cu, PAA and LA. For expression analysis by qRT-PCR, 10 day old seedlings of IR64 variety (a moderately salt-sensitive cultivar) of rice were subjected to stress treatment for 8 hrs followed by RNA isolation, first strand cDNA synthesis and real-time PCR. Error bars showed standard deviation of three biological replicates.