Fig. 1.
P4pal-10 is an antagonist of multiple Gq-coupled receptors. (A) Agonist-promoted calcium mobilization was monitored by Fura-2 AM fluorescence in HEK293 and HASM cells pretreated for 1 minute with various concentrations of P4pal-10. Peak fluorescence values are reported. (B) P4pal-10 IC50 values are reported for responsiveness to various Gq-coupled receptor agonists. (C) Ten micromolars of P4pal-10 does not inhibit isoproterenol-stimulated cAMP production (Gs mediated) in HEK293 cells. The data are represented by the mean ± S.D. from three independent experiments; ns, not significant. (D) Ten micromolars of P4pal-10 does not inhibit SDF-1α–stimulated adenylyl cyclase inhibition (Gi mediated) in HEK293 cells. The data are represented by the mean ± S.D. from three independent experiments. **P < 0.01 versus forskolin-treated cells using a two-way unpaired t test.