Fig. 1.

Differential recruitment of coregulators to ERα upon CE, BAZ, and TSEC treatment. (A) ERα/ERE-DNA pull-down analyses were performed using HeLa nuclear extracts in the presence of vehicle, CE (10 nM), BAZ (100 nM), and TSEC (10 nM CE plus 100 nM BAZ) as described in Materials and Methods. Proteins associated with ERα after different hormone treatments were identified by MS analyses, and the peptide numbers were listed. (B) After ERα/ERE-DNA pull-down analyses, the levels of SRC-1, SRC-2, SRC-3, BCOR, and ERα in the precipitates were determined by Western blotting analyses. SRC-1 (C), SRC-2 (D), and SRC-3 (E) protein levels in HeLa cells treated with hormone for 24 hours were determined by Western blotting analyses (n = 3/group). The tubulin level in each group was used as a protein loading control. (F) The ratios of SRCs to tubulin levels in (C)–(E) are shown in a graph. NS, nonspecific, Student’s t test. Values represent the average ± S.E.M. of three independent experiments.