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. 2016 Jan;89(1):14–26. doi: 10.1124/mol.115.100925

Fig. 5.

Fig. 5.

FBXO45 involves in TSEC-mediated ERα degradation. (A) HeLa cells were transfected with an ERα expression vector and different amounts of expression vectors for Flag-tagged FBXO45. The ERα, FBXO45, PR, and tubulin protein levels in each group were determined by Western blotting analyses. (B) HeLa cells transfected with ERα were treated with 100 nM nontargeting siRNA or FBXO45 siRNA for 3 days followed by vehicle, CE (10 nM), BAZ (100 nM), and TSEC (10 nM CE and 100 nM BAZ) for 24 hours. Subsequently, the ERα, FBXO45, and tubulin protein levels in each group were determined by Western blotting analyses. (C) HeLa cells were transfected with expression vectors for ERα and FBXO45. The cells were then treated with vehicle, 10 nM CE, 100 nM BAZ, or TSEC (10 nM CE plus 100 nM BAZ) for 0, 1, 3, 6, and 9 hours. Subsequently, the ERα, FBXO45, and tubulin protein levels in each group were determined by Western blotting analyses. (D) Quantification of ERα levels in panel C is shown in the graph. hs, hours; RF, relative fold compared with control.