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. 2016 Jan;89(1):154–164. doi: 10.1124/mol.115.100255

Fig. 3.

Fig. 3.

CYP2C8, but not CYP2C9, protein levels were induced by BF in human primary hepatocytes. Human primary hepatocytes isolated from between three to five different donors were treated with either DMSO control or 0.5 mM BF for 48 hours. Microsomes prepared from DMSO control or BF-treated cells were analyzed by Western blot for CYP2C proteins. The figure is a representative blot. HLM, human liver microsomes (Gentest, Woburn, MA). The following recombinant yeast CYP2C protein controls were used: CYP2C9 (2C9), CYP2C8 (2C8), and CYP2C19 (2C19). 1590 is an anti-CYP2C antibody, whereas 1937 is a specific antibody for a CYP2C8 peptide that does not crossreact with other human CYP2C proteins. The specificity of the antibodies is presented in Materials and Methods. (B) Densitometry analysis of Western blot using Image J software. Data indicate the mean ± S.E.M. calculations of a minimum of three independent donor preparations. ***P < 0.001 compared with DMSO control (Student’s t test).