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. 2016 Jan;89(1):154–164. doi: 10.1124/mol.115.100255

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Fig. 5. — PPARα-regulated transcription of CYP2C8 is mediated by a PPRE (DR1-B) located at −2109 bp upstream of the translation start site. (A) Computer analysis of the CYP2C8 promoter showed five putative PPRE sites located at −2772 (DR1-A), −2109 (DR1-B), −2039 (DR1-C), −1501 (DR1-D), and −152 (DR1-E) bp relative to the translation start site. (B) HepG2 cells were transiently transfected with various luciferase constructs containing the indicated sequences of the CYP2C8 promoter cotransfected with hPPARα expression plasmids. Twenty-four hours after transfection, cells were treated with either DMSO (0.1%) or BF (0.5 mM) for 24 hours. Cells were harvested, and luciferase and Renilla luciferase activity were measured. The results were expressed as a fold induction compared with DMSO-treated cells. ***P < 0.001 compared with DMSO-treated cells (one-way analysis of variance followed by Tukey’s test).