Skip to main content
. 2016 Jan 6;6:18769. doi: 10.1038/srep18769

Figure 3. EMB directly interacts with NS conformers.

Figure 3

(A–D) SEC profiles for identified NS conformers after overnight incubation (1.7 mg/ml) in the presence or absence of EMB at 45 °C; A: Nat, B: Pol, C: Lat, and D: Cle. The continuous black line corresponds to NS in the absence of EMB, monitored at 280 nm; the dashed black line and the red line are the SEC profiles after NS incubation with EMB, monitored at 280 nm and at 340 nm respectively. (E) Absorption spectra of a 8.2 μM NS solution (green line) and of a 60 μM EMB solution (red line); Nat incubated for two hours in saturated EMB solution and then washed to remove free EMB (black line); difference spectrum between the black spectrum and the previous two spectra (Nat and EMB solutions) (blue line). Inset: difference spectra calculated as above at different NS concentrations. Each spectrum is normalised for NS concentration.