Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 6;6:18846. doi: 10.1038/srep18846

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) 293T REx-RRM2B or-Flag-RRM2B cells were treated with DOX and lysed after 24 hours. WCL was precipitated with Flag antibody. (B) Proliferating 293T cells were lysed and WCL was immunoprecipiated with antibodies to RRM2B or control rabbit IgG (C) WCL from 293T was immunoprecipitated with antibodies to PYCR1, PYCR2 or control rabbit or mouse IgG. (D) 293T cells were co-transfected with either RRM2B or Flag-RRM2B vectors and HA-PYCR1, or HA-PYCR2 vectors and lysed 48 hours later. Whole cell lysates were subjected to immunoprecipitation with antibodies to HA or Flag. Denatured whole cell lysates and immune complexes electrophoretically separated on gels were transferred to membranes and blotted with antibodies to RR subunits, PYCR1, PYC2, HA and Flag. (E) HFF-hTERT cells transduced with retroviruses expressing Flag-RRM2B were fixed and analyzed by indirect immunofluorescence. RRM2B (far red and pseudo-colored with green; left panel) and PYCR2 (red; middle panel) were visualized by confocal microscopy. Co-localization of RRM2B and PYCR2 was observed in the merged image (right panel).