Figure 5. Silencing of PYCR1 and PYCR2 Induced Hypersensitivity to H₂O₂ in HFF-hTERT Cells.

(A) HFF-hTERT cells were transduced with pSUPER.retro.puro retroviruses expressing shRNAs silencing both PYCR1 and PYCR2 or non-silencing (NS) control. Cells were seeded at the low density four days post-infection and treated with 200 μM H₂O₂ for 10 minutes next day. Colonies were stained and total colony numbers were scored after two weeks. Data were plotted as % of shNS control or (B) surviving fraction (SF) after treatment of 200 μM H₂O₂. (n = 3) (C) HFF-hTERT cells were transduced with pLKO.1 lentiviruses expressing shRNAs silencing both PYCR1 and PYCR2 or non-silencing (NS) control. Cells of equal number were seeded at the low density four days post-infection. Cells were treated with 50 μM H₂O₂ for 10 minutes the next day and cell numbers were scored after two days. Data were plotted as % of shNS control or (B) surviving fraction (SF) after treatment of 50 μM H₂O₂. (n = 3).