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. 2016 Jan 6;6:18850. doi: 10.1038/srep18850

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(A) Original image of a stage-4 egg chamber. (B) Detected mask of nurse cells. (C) Detected nurse cells in original intensities. (D) Watershed algorithm output. Each nurse cell had been further divided into several small parts denoted by different colors. This varied coloration indicates the existence of blob-like chromosomes in polytene nuclei. (E) Original image of a stage-10B egg chamber. (F) Detected mask for inside cells. (G) Possible centripetal migration region was highlighted as adjustable black band perpendicular to the major axis. In this picture, part of two nurse cells were captured in this band (red arrow), and since they only partially belonged to this band, the algorithm excluded those as candidates of centripetal migration cells. (H) Detected centripetal cell migration. Centripetal cell migration was identified as whole cells inside the band near the outer boundary.