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. 2016 Jan 6;6:18945. doi: 10.1038/srep18945

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Copyright © 2016, Macmillan Publishers Limited

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(a) In situ hybridization for VEGF mRNA in the calcitriol and control groups (original magnification × 400). (b) The integral absorbance (IA) values for VEGF mRNA were 366624.00 ± 50300.32 in the calcitriol group and 238306.20 ± 43730.12 in the control group. (c) VEGF expression in each group as assessed by immunohistochemistry (original magnification × 200). (d) The IA values of VEGF protein were 82087.16 ± 12687.08 in the calcitriol group and 50490.62 ± 8883.89 in the control group. (e) Protein expression of VEGF in each group, as assessed by Western blot analysis. The gels have been run under the same experimental conditions, and cropped blots are used here. The full-length gel images are available in Supplementary Fig. 3e. (f) Densitometry results of VEGF protein expression in the two groups. Values are expressed as means ± SEMs, n = 6 per group. **p < 0.01, vs. control group.