Table 1. Virtual screening hits and their in vitro assay results for mTOR inhibitions.

Compound	% of mTOR inhibition at 10 μMa	IC50(μM)b
6	22%	ND
9	23%	ND
12	30%	ND
13	76%	5.83 ± 1.85
17	61%	7.59 ± 2.44
18	38%	>50
20	55%	9.20 ± 2.15
21	50%	14.38 ± 2.02
25	36%	>50
26	33%	>50
27	38%	28.65 ± 2.65
33	21%	ND
36	31%	14.88 ± 1.01
37	22%	ND
40	91%	1.47 ± 0.33
Wortmaninc	–	0.14 ± 0.02

^a% Inhibition values are the mean ± SD of triplicate measurements at 10 μM.

^bIC₅₀ values for mTOR shown are the mean ± SD of triplicate measurements.

^cUsed as a positive control compound. ND: not determined. Wortmanin is suggested and supplied by CBA104-1KIT from Calbiochem.