Figure 3. Wounding and simulated herbivory change the transcript levels of various GmCDPKs.

Plants were wounded with a pattern wheel, and 20 μL of water (W+W) or S. exigua oral secretions (W+OS) were immediately applied to the wounds; untreated plants served as controls. Individual leaves from 5 replicate plants were harvested 1.5 h after the treatments, and GmCDPKs’ expression levels were analyzed by q-PCR. (A) Ratios (mean ± SE) between the GmCDPK transcript levels (Log₂ transformed) in W+W- or W+OS-treated and untreated controls. Asterisks indicate significant changes (t-test, p < 0.05) of more than 1.5-fold up- or down-regulated genes. (B) Venn diagram depicting the number of genes having similar or differential (t-test, p < 0.05) expression in response to W+W and W+OS treatments.