Table 3. Proximity of the lethal genes to markers located within the regions linked to HN in the Pyrus PEAR3 × ‘Moonglow’ population.
| LG5 PEAR3 | ||||
|---|---|---|---|---|
| Marker | CH03a09 | LETss527789863 | Hi04d02 | CH05f06 |
| Position (cM) | 9.2 | 26.2 | 48.3 | 56.2 |
| Segregation | 〈lmxll〉 (115:117 × 115:115) | 〈abxcd〉 | 〈abxcd〉 (164:173 × 158:197) | 〈abxcd〉 (156:181 × 173:179) |
| Phase | {1−} | {01} | {11} | {00} |
| Incompatible allele | l (115 bp) | b | a (164 bp) | b (181 bp) |
| % of ‘Type 1’ bringing the incompatible allele | 48.8 | 90.7 | 83.7 | 81.3 |
| LG1 ‘Moonglow’ | ||||||||
|---|---|---|---|---|---|---|---|---|
| Marker | LETss527788115 | MDP0000711403_LG1d | MDP0000711403_LG1a | LETss527789610 | MDP0000160413_LG1b | CHVf1 | MDP0000251943_LG1b | MDP0000508070_LG1b |
| Position (cM) | 28.9 | 40.8 | 42.5 | 49.1 | 50.3 | 51.0 | 58.4 | 71.9 |
| Segregation | 〈nnxnp〉 | 〈nnxnp〉 | 〈nnxnp〉 | 〈abxcd〉 | 〈nnxnp〉 | 〈nnxnp〉 (129:129 × 127:0) | 〈abxcd〉 | 〈nnxnp〉 |
| Phase | {−1} | {−0} | {−1} | {10} | {−1} | {−0} | {10} | {−0} |
| Incompatible allele | n | p | n | d | n | p (0) | d | p |
| % of ‘Type 1’ bringing the incompatible allele | 76.9 | 81.8 | 83.6 | 85.2 | 85.2 | 86.5 | 77.8 | 47.2 |
| LG2 PEAR3 | ||||||
|---|---|---|---|---|---|---|
| Marker | CH02f06 | Hi08g12 | CN493139 | LETss527788384 | CN444636 | Hi24f04 |
| Position (cM) | 0.0 | 4.5 | 14.6 | 27.7 | 28.2 | 31.7 |
| Segregation | 〈abxcd〉 (150:0 × 174:177) | 〈efxeg〉 (179:196 × 179:205) | 〈efxeg〉 (138:148 × 135:148) | 〈lmxll〉 | 〈abxcd〉 (243:253 × 237:245) | 〈lmxll〉 (129:139 × 144:144) |
| Phase | {01} | {01} | {11} | {0−} | {11} | {0−} |
| Incompatible allele | b (0) | f (196 bp) | e (148 bp) | m | a (243 bp) | m (139 bp) |
| % of ‘Type 2’ bringing the incompatible allele | 70.8 | 84.7 | 87.8 | 87.6 | 87.7 | 83.6 |
For the combined loci from LG5 of PEAR3 and LG1 of ‘Moonglow’, the percentage of ‘Type 1’ contributing the incompatible alleles over the total ‘Type 1’ seedlings genotyped was calculated. For LG2 of PEAR3, the percentage of ‘Type 2’ contributing the incompatible alleles over the total ‘Type 2’ seedlings genotyped was calculated. The higher the percentage, the closer the marker is to the lethal gene. For each marker, the location on the genetic map, the allelic composition, linkage phase (with respect to the parent where the marker was mapped) and the incompatible allele are shown. The closest marker to the lethal gene is indicated in bold.