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. 2016 Jan 6;6:18738. doi: 10.1038/srep18738

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(a) Representative Ca²⁺ signaling images in the spleen of a YC3.60^flox/CD19-Cre mouse are shown. A rainbow parameter indicates relative Ca²⁺ concentration. (b) Time courses for fluorescence intensities of CFP and YFP (Venus) and the YFP/CFP ratio at excitation of 458 nm in indicated cells in (a) are shown. Scale bar, 25 μm. Frame = 80.