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. Author manuscript; available in PMC: 2016 Jan 6.
Published in final edited form as: Autoimmunity. 2010 Sep 9;43(8):682–689. doi: 10.3109/08916934.2010.506207

Figure 6.

Figure 6

Effect of SLE-ITP patients Abs on platelet fragmentation in vitro and in vivo. (A) Presence of platelet fragments in SLE-ITP patients. Serum immune complexes from GPIIIa49-66 positive patients or healthy subjects run on 12% SDS-PAGE (upper) and immunoblotted with anti-GPIIIa49-66 antibody (lower). (B) Effect of affinity-purified anti-GPIIIa49-66 IgG from three SLE-ITP patients on platelet fragmentation in vitro. Pt refers to anti-GPIIIa49-66 Ab from HIV-ITP patients. White, dark, and gray bars refer to serial 1:1 dilutions of IgG, starting at 50 μg/ml, SEM is given. (C) Effect of affinity-purified anti-GPIIIa49-66 antibodies from Patient P7 on induction of thrombocytopenia in Balb/C mice. Purified control antibody (Ctl), patient anti-GPIIIa49-66 antibodies (50 μg) were injected intraperitoneally into Balb/C mice, and platelet counts followed for 24 h, n = 4, SEM is given.