Table 1.
Primers covering gag, pol, LTR and env regions, were used for the SRLV detection by PCR
| PCR assay | Primers | Sequence 5′–3′ | References |
|---|---|---|---|
| Gag nested PCR | GAG F1 | TGG TGA RKC TAG MTA GAG ACA TGG | [64] |
| GAG F2 | CAA ACW GTR GCA ATG CAG CAT GG | ||
| POL R1 | CAT AGG RGG HGC GGA CGG CAS CA | ||
| POL R2 | GCG GAC GGC ASC ACA CG | ||
| Pol nested PCR | P28 | CAT GAA GAG GGG ACA AAT CAG CA | [18] |
| P34 | TAC ATT GGG TGC CTG GAC ATA A | ||
| P32 | TAC CTG DGT TGG TCC YWG CCA CT | ||
| P33 | CTT CCC AVA GTA CCT GDG TTG GTC | ||
| P29 | GGT GCC TGG ACA TAA AGG GAT TC | ||
| P35 | GCC ACT CTC CTG RAT GTC CTC T | ||
| Gag-Can nested PCR | CAEV F0 | AAC TGA AAC TTC GGG GAC GCC TG | [5] |
| CAEV F1 | AAG GTA AGT GAC TCT GCT GTA CGC | ||
| CAEV F2 | TGG TGA GTC TAG ATA GAG ACA TGG | ||
| CAEV R0 | GTTATCTCGTCCTAATACTTCTACTGG | ||
| CAEV R1 | TTT TTC TCC TTC TAC TAT TCC YCC | ||
| CAEV R2 | GGA CGG CAC CAC ACG TAK CCC | ||
| MVV F0 | AAG TAA GGT AAG AGA GAC ACC TAC TGG | ||
| MVV F1 | TAG ATA GAG ACA TGG CGA AGC AAG CTC | ||
| MVV F2 | GAC AGA AGG GAA CTG TCT ATG GGC | ||
| MVV R0 | GGT GGT GCT TCT GTT ACA ACA TAG G | ||
| MVV R1 | GGA CGG CAC CAC ACG TGG | ||
| MVV R2 | CCC CTC CTG YTG TTT CCC TG | ||
| BIV nested PCR | POL3 | ACA ACG GGC CGT GCT TTA CTG | [65]; [66] |
| POL4 | CCT CTT CCT CTA TTA CTG CTG C | ||
| POL5 | GAR AAT CTA TGT AAG TAT CTG GG | Unpublished data (Nadin-Davis) | |
| POL6 | CTG TTY CTT ACG TAA CAC CAC T | ||
| P09 | CAC TGG ACG AGA TGA GGT AGT | [67] | |
| P06 | TGG TAG TCT GAT AAA TGG CA | ||
| P04 | CAG GCT CTT AAG GAA ATT GT | ||
| P11 | CCA TCC TTG TGG TAG AAC ATT | ||
| Craft-Oslo | Craft-Fw | TGA CAG AAG GAA ATT GTY TRT GG | |
| Oslo-Rv | GGC ATC ATG GCT AAT ACT TCT AA | [68] | |
| LTR PCR | LTR-Fw | TGA CAC AGC AAA TGT AAC CGC AAG | [69] |
| LTR-Rv | CCA CGT TGG GCG CCA GCT GCG AGA | ||
| CO qPCR | 612-F | GAA CTC AGC CAC AAG AGG AAG AA | [70] |
| 613-R | CCT GCG GCA GCT ACT ATT GC | ||
| 614 Probe | FAM AACTAGCATAATGACCAAGCCAACGCCTCT BHQ-1 | ||
| A4 qPCR | Fs7631/Fg6221 | ACA AAC TGG ACC ACC ATG CA | [21] |
| Rg6221 | CTA GTG TTC CAT TTC CTG TTC TGT TTA | ||
| Probe g6221 | FAM GGC AAC TGT TCW CAG TGT GTT AAT GYA ACG BHQ-1 | ||
| A4-SU PCR | SU5-1F | GTA GAT GTG TAC AAA GAC CAG | [21] |
| SU5-1R | CTG CCT CTA ACA CTT GCT GC | ||
| CO-SU PCR | B1-SU5-1R | TGC CTC TAA CAC ATC CTG CTG TGC | [70] |
| B1-SU5-1F | GGT GGA ACA TAT GAC AGG AG |
Primers able to amplify bovine inmunodeficiency virus were included