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. Author manuscript; available in PMC: 2016 Mar 11.
Published in final edited form as: Science. 2015 Sep 11;349(6253):1216–1220. doi: 10.1126/science.aab3415

Fig. 1. Er81 expression distinguishes PV+ basket cell n subtypes.

Fig. 1

(A) Er81 protein expression in the somatosensory cortex. (B) PV+/Er81+ interneurons are particularly abundant in cortical layer II-III, with a superficial to deep gradient within this layer (n = 11 P25 mice). (C and D) Representative traces of firing at near threshold potential for Er81+ (red) and Er81− (gray) PV+ interneurons. NB, Neurobiotin. (E) Mean latency to the first spike (n = 22 and 9 cells, respectively; P < 0.001). (F) Individual factor maps from principal component analysis performed on recorded cells reveals a significant segregation between Er81+ and Er81− PV+ interneurons. (G) Phase plane plots for Er81+ and Er81− PV+ interneurons. (H) Threshold potential for spike is significantly different between Er81+ and Er81− PV+ interneurons (n = 21 and 11 cells, respectively; P < 0.05). (I and K) Schematic of mEPSCs and mIPSCs recordings in PV+ interneurons. PC, pyramidal cell; PV, PV+ interneuron; IN, Interneuron. (M and N) Representative traces. (J and L) Measurements of mEPSC (n = 15 and 6 cells, respectively; P < 0.05) and mIPSC (n = 7 and 4 cells, respectively; P < 0.05) frequencies in PV+ interneurons. Scale bars, 100 μm (A) and 10 μm (C) and (D). Graphs represent means ± SEM.