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. Author manuscript; available in PMC: 2016 Mar 11.
Published in final edited form as: Science. 2015 Sep 11;349(6253):1216–1220. doi: 10.1126/science.aab3415

Fig. 3. Reduced excitatory inputs onto PV interneurons in conditional Er81 mutants.

Fig. 3

(A) Schematic of mEPSCs recordings in PV+ interneurons. (B) Representative traces. (C and D) Measurement and cumulative plot of mEPSC frequencies in Lhx6-Cre;Er81F/F (n = 11 and 9 cells, respectively; P < 0.05) and PV-Cre;Er81F/F (n = 17 and 10 cells, respectively; P < 0.01) mutants. (E) Measurement of mEPSC amplitudes in Lhx6-Cre;Er81F/F (n = 11 and 9 cells, respectively; P < 0.05) and PV-Cre;Er81F/F mutants (n = 17 and 10 cells, respectively; P = 0.06). (F) Experimental design for the labeling of sparse control and Er81 mutant MGE-derived interneurons. (G) Labeled interneurons are sparsely distributed throughout the cerebral cortex at very low density. (H and I) Representative images of layer II-III PV+ interneurons after viral infection in the MGE. (J) Representative traces of mEPSCs from control and Er81 mutant interneurons. (K) Cumulative plot of mEPSCs frequencies (n = 10 and 11 cells, respectively; P = 0.45). (L) Measurement of mEPSC amplitudes (n = 10 and 11 cells, respectively; P < 0.05). Scale bars, 10 μm and 5 μm (G) and (H) and 100 μm (I). Graphs represent means ± SEM.