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. 2016 Jan 5;16:4. doi: 10.1186/s12870-015-0698-8

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© Kato et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Analysis of phytoene production in E. coli by HPLC. HPLC chromatogram (284 nm) of extracts from E. coli carrying a pET-EgcrtE with pAC-PacrtB, b pACCRT-E [23] with pET-EgcrtB and c pET-EgcrtEB. d Absorbance spectrum of phytoene detected at a retention time of 28.6 min. Phytoene was extracted from E. coli transformants and analyzed with HPLC in accordance with the method of Takaichi [57]. The arrowheads in the chromatograms indicate the position of phytoene elution. Data are representative of three or four experiments with similar results