Fig. 10.

Apc knockout in lung mesenchyme disrupted pulmonary vascular network formation and pulmonary circulation continuity. a The endothelial cells of E13.5 pulmonary vasculature that was connected to the right heart were labeled with FITC-lectin after its intracardial injection, while all mature vascular endothelial cells were immunostained with anti-PECAM1. b Decreased expression of Flk1 at the mRNA level was readily detected in E12.5 Apc CKO lungs, *P < 0.05, n = 5. c Dynamic changes of angioblasts (Flk1⁺/PECAM1⁻) and mature endothelial cells (Flk1⁺/PECAM1⁺) in Apc CKO lung. Reduced angioblasts were detected in Apc CKO lung at E13.5, while both angioblasts and mature endothelial cell numbers appeared significantly reduced in Apc CKO lung at E14.5. d Increased Ctnnb1 activation was not detected in Flk1⁺ cells in Apc CKO lung. e Reduced expression of Igf1 and Angpt1 at the mRNA level was detected in Apc CKO lungs at E12.5 (*P < 0.05, n = 5)