Fig. 8.

Hyperactivation of Wnt/Ctnnb1 pathway caused by Apc deletion in lung mesenchyme induced abnormal expression of Vcan. a Increased Vcan protein expression and distribution were detected in Apc CKO lung after E12.5, shown by immunofluorescence staining. Cell nuclei were counterstained with DAPI (blue). b Simultaneous deletion of Ctnnb1 in E13.5 Apc CKO lung mesenchyme blocked excessive Vcan expression. c Two sites containing Ctnnb1 binding DNA consensus sequences, which have been reported in Ctnnb1 target gene Axin2, were identified in Vcan promoter DNA. d Interaction between Ctnnb1 and Vcan promoter DNA was validated by ChIP using anti-active Ctnnb1 antibody. Anti-histone H3 and normal IgG were used as positive and negative controls, respectively