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. Author manuscript; available in PMC: 2016 Dec 16.
Published in final edited form as: Neuron. 2015 Nov 29;88(6):1136–1148. doi: 10.1016/j.neuron.2015.10.032

Figure 5. Multilayered Control of Spiking.

Figure 5

(A) Spiking of a PYR (red triangle) and an inter-neuron (blue circle) recorded from the CA1 pyramidal cell layer of a freely moving CaMKII::ChR2 during illumination (4.2 μW, 30 cycles) within the layer (μLED1) and at more distant loci. Stratum oriens (μLED2) illumination induced more robust spiking than putative somatic illumination (μLED1).

(B) Dependence of spike timing on light power (abscissa) and μLED-soma distance. Left: PYR spiking offset (relative to time of peak light power) depends on μLED-soma distance. Numbers show circular-linear correlation coefficients; error bars, SEM; */***p < 0.05/0.005, permutation test; n = 38 PYR. Right: temporal jitter (SD of spike timing) consistently depends on light power but inconsistently on μLED-soma distance. Only distance-power bins in which the number of time-locked units exceeded chance (p < 0.05, Binomial test) are shown. Numbers, rank correlation coefficients. See also Figure S5.