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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Transl Res. 2015 Aug 29;166(6):740–749.e1. doi: 10.1016/j.trsl.2015.08.007

Figure 1. Derivation of iPSCs from a patient with clinically diagnosed LCA.

Figure 1

A–C: Microscopic analysis of iPSC cultures generated from a 3 year-old patient with clinically diagnosed LCA. At 2–3 weeks post-viral transduction iPSC colonies that were isolated, subcultured, and clonally expanded on SynthemaxTM cell culture surfaces maintain a pluripotent morphology (A, B), and express the pluripotency markers SSEA4 as determined by live staining (B, inset) and DNMT, LIN28, OCT4, SOX2, NANOG, C-MYC, and KLF4 as determined by RT-PCR (C). Scale bar = 1mm (A), 400um (B).