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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Transl Res. 2015 Aug 29;166(6):740–749.e1. doi: 10.1016/j.trsl.2015.08.007

Figure 2. Generation of iPSC-derived RPE cells from a patient with clinically diagnosed LCA.

Figure 2

A–B: iPSC-derived eyecup-like structures generated from a patient with clinically diagnosed LCA (A, low magnification – B, high magnification). C–E: Following isolation, dissociation and subculture (C), pigmented cells adhere to the cell culture surface, spread, lose pigmentation, and assume a fibroblastic morphology (D). By 3 weeks post-plating, a confluent monolayer of RPE cells are present that have taken on the typical cuboidal RPE morphology and regained pigmentation (E). F: RT-PCR analysis using primer pairs targeted against the RPE transcription factors PAX6 and MITF, the tight junction marker ZO1, and the RPE-specific channel BEST1. G–I: Immunocytochemical analysis using antibodies targeted against the RPE transcription factors PAX6 (G) and MITF (H), and the tight junction marker ZO1 (I).