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. 2015 Dec 17;90(1):254–265. doi: 10.1128/JVI.01720-15

FIG 4.

FIG 4

Effects of cyclic dinucleotides on genotype 2a JFH1 replicon and infectious virus replication. (A) Effects of cyclic dinucleotides on 2a/JFH1 replicon replication. Huh7.5 cells were electroporated with in vitro-transcribed replicon RNA and seeded onto a 96-well plate. After 24 h, the medium was changed and the cells were treated with indicated concentrations of cyclic dinucleotides for an additional 48 h. Luciferase activity was measured, and the data were plotted relative to the mock-treated control. The data represent two independent experiments, each with triplicate samples. The open circle and triangle represent c-di-GMP and cGAMP, respectively. The dashed line represents the result from 1b/Con1 replicon, which served as the control. (B) Effects of cyclic dinucleotides on JFH1 infectious virus replication. Huh7.5 cells were infected with JFH1 virus at an MOI of 0.01 FFU/cell for 4 h and then treated with indicated concentrations of cyclic dinucleotides. Viral RNA was quantified by real-time RT-PCR 48 h posttransfection and normalized to GAPDH. The data represent the percentage of virus replication compared to mock-treated sample.