FIG 1.

Schematic design of the recombinant viruses and quantification of surface glycoproteins in purified virions. (A) RSV genome with G gene open reading frame (amino acids 1 to 298) enlarged to illustrate the mutations made to generate the Gstop virus. The two methionines were changed to isoleucine, and the second codon (serine) was changed to a stop codon. (B) Western blot showing the F, G, and N protein expression levels (left) and densitometry combined from the results of four independent experiments (right). Data are represented as means ± SEM.