FIG 1.

Screening of EBV lytic genes to identify potential MHC class I immune evasion genes. MJS cells were transiently transfected with pCDNA3.1-IRES-GFP plasmids carrying a selection of EBV lytic genes. At 24 h posttransfection, surface levels of MHC class I (A) and MHC class II (B) on GFP-positive cells were analyzed using two-color flow cytometry. (C) AKBM cells were induced into the lytic cycle by cross-linking of B cell receptors for 1 h at 37°C and were analyzed by Western blotting at the indicated time points postinduction. Levels of BZLF1 (upper blot), BDLF3 (middle blot; stars indicate monomeric and trimeric forms of BDLF3), and the loading control calregulin (lower blot) are shown.