FIG 3.

Effect of differentiation status on susceptibility to HRV infection. (A) Undifferentiated and differentiated jejunal enteroids from the same patient were either mock infected or infected with HRV Ito at an MOI of 10 FFU/cell. At 20 hpi, single-cell suspensions were assessed for the presence of intracellular rotavirus antigen by flow cytometry. Results are representative of data from triplicate independent experiments. Each data bar represents the mean ± standard deviation for 3 samples within each independent experiment. (B) Undifferentiated and differentiated jejunal enteroids from the same patient (patient j2) were infected with HRV Ito at an MOI of 0.5 FFU/cell, and the amount of infectious virus was quantified at 1.5 hpi (light gray bars) and 24 hpi (dark gray bars). Fold increases are displayed above the bars. Results are representative of data from replicate experiments performed with enteroids from two additional patients (patients j3 and j11). Each data bar represents the mean ± standard deviation for 4 samples within each independent experiment. Statistical analyses were performed by using Student's t test.