FIG 5.

Both YAP and MRTF-A are required for induction of multiple genes. (A to D) Glioblastoma cells were transfected with siYAP, siMRTF-A, or both for 48 h. Cells were serum starved for 24 h and then treated with 0.3 μM S1P for 1 h. Total cell lysates were subjected to qPCR analysis of mRNA levels for CCN1, CTGF, ANKRD1, or ACTA2. **, P < 0.01 versus siCon (n = 3). (E to H) The experiments for panels A to D were repeated with a second siRNA construct for YAP and a second siRNA construct for MRTF-A. **, P < 0.01 versus siCon (n = 3).