FIG 3.

Syn1-Cre-mediated Brg1 deletion in neurons impairs synapse maturation in vivo. Individual DG granule neurons in fixed P21 Brg1^F/F and Syn1-Cre Brg1^F/F hippocampal slices were injected with Lucifer yellow to enable visualization. (A) Representative two-photon confocal microscopy images of dendritic spines. Scale bar, 5 μm. (B to D) Average spine densities (B), classifications of spines (C), and average spine volumes from Brg1^F/F (n = 18) and Syn1-Cre Brg1^F/F (n = 20) neurons (D) are shown. Values in the graphs are averages plus standard errors. *, P < 0.05 (Student's t test).