FIG 4.

Brg1 regulates synaptic genes in developing neurons. (A) Brg1-regulated genes in P13 and P21 DG identified with RNA-seq were compared. The Venn diagrams show the numbers of common genes activated or repressed by Brg1 in each developmental stage. (B) Examples of neuronal genes activated and repressed by Brg1 in both P13 and P21 DG. (C) Brg1 ChIP-qPCR experiments were performed using P13 and P21 DG. Enrichment of Brg1 binding to the regulatory regions of the indicated target genes compared to results with IgG ChIP was observed. A region in the CD4 gene was used as a Brg1 binding negative control. Values in the graph are shown as averages plus standard errors (n = 3). (D) List of the most enriched Brg1-regulated biological processes identified by DAVID gene ontology analysis of the RNA-seq data set obtained from both P13 and P21 DG. (E) Significant overlap between Brg1-regulated genes in developing DG and synaptic genes (52) and autism genes (SFARI gene database). Fisher's exact test was used to calculate P values.