FIG 5.

BAF53b-Cre-mediated Brg1 deletion in cultured cortical/hippocampal neurons. (A) BAF53b-Cre was used to mediate Brg1 deletion in cultured neurons. Mixed cortical and hippocampal neurons cultured from E18.5 embryos with the indicated genotypes at 1 DIV, 7 DIV, and 14 DIV were stained with antibodies against Brg1, YFP reporter, and the neuronal marker HuC/D. Note that at 1 DIV, Brg1 was not completely deleted in YFP-labeled neurons in Brg1 mutant culture, whereas at 7 DIV and 14 DIV, Brg1 was completely deleted in YFP⁺ Brg1 mutant neurons. At 14 DIV, the YFP reporter completely costained with all HuC/D-positive cells, confirming the pan-neuron-specific feature of BAF53b-Cre transgene. DAPI, 4′,6′-diamidino-2-phenylindole.