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. 2015 Dec 18;36(1):30–38. doi: 10.1128/MCB.00702-15

FIG 3.

FIG 3

The miR-140 binding sites within mouse NEAT1 (mNeat1) were identified using RNAhybrid 2.2. (A) Target binding sites of miR-140 (BS1 and BS2) within NEAT1. (B) Luciferase reporter assays were performed and confirmed that ectopic expression of miR-140 increased the activity of the BS1 reporter. (C) Ectopic expression of miR-140 has no impact on BS2. (D) Mutation of BS1 inhibited miR-140 induction of luciferase activity. (E) Biotin-labeled miR-140 pulldown demonstrated binding of miR-140 to NEAT1. ACTB, β-actin. The data represent means and SE (n = 3). *, P < 0.05.