TABLE 1.
Effects of redox perturbation of NADH and NADPH levels on extracellular metabolite production at the end of fermentations and on biomassa
| Acetoin added (mM) for indicated strain | Glycerol (mmol/g DW) | Ethanol (g/liter) | Acetate (mmol/g DW) | Succinate (mmol/g DW) | Pyruvate (mmol/g DW) | Biomass (g/liter) |
|---|---|---|---|---|---|---|
| 59A-NADH-Bdh | ||||||
| 0 | 16.5 ± 0.19 Bb | 97.9 ± 0.59 B | 2.89 ± 0.11 A | 0.95 ± 0.09 B | 0.17 ± 0.03 A | 3.98 ± 0.07 C |
| 200 | 10.6 ± 0.23 A | 92.2 ± 1.85 A | 14.7 ± 0.43 C | 1.04 ± 0.08 B | 0.40 ± 0.03 B | 3.11 ± 0.08 A |
| 59A-NADPH-Bdh | ||||||
| 0 | 19.0 ± 0.51 C | 98.1 ± 1.69 B | 3.39 ± 0.08 A | 0.96 ± 0.05 B | 0.22 ± 0.06 A | 3.86 ± 0.15 C |
| 200 | 16.9 ± 0.32 B | 96.2 ± 0.68 B | 9.08 ± 0.16 A | 0.59 ± 0.02 A | 0.30 ± 0.02 AB | 3.38 ± 0.04 B |
a
The experiments were performed in SM250 containing 100 g/liter glucose and 100 g/liter fructose. Concentrations were measured at the end of fermentations, after the exhaustion of sugars.
b
Different letters denote significant differences between the treatments at P < 0.05, as determined by ANOVA followed by Tukey's test.