FIG 4.

Biochemical characteristics of rAly5 and its truncated enzyme rAly5-T336N. (A) Effects of temperature on enzyme activities. (B) Enzyme thermostabilities. The enzymes were preincubated at pH 6 for 2 h at different temperatures. (C) Effects of pH on enzyme activities. (D) Stabilities of enzymes as a function of pH. The enzymes were preincubated for 2 h at 0°C under different pH conditions. The residual activities were determined under optimal temperature conditions. (E) Effects of various compounds on the enzyme activity of rAly5.