FIG 6.

Oligosaccharide degradation patterns of rAly5 and its truncated protein T336N. (A) Degradation of unsaturated oligosaccharides (∼20 μg) by rAly5 (A) at 40°C for 12 h. E (−), without the enzyme; E (+), with the enzyme at 0.1 U/ml. HPLC analyses were performed using a Superdex peptide 10/300 GL column monitored at a wavelength of 235 nm. (B) Degradation of 2-AB-labeled unsaturated pentosaccharides (2-AB-UDP5) by rAly5 and rAly5-T336N. Fluorescent HPLC analyses were performed using a Superdex peptide 10/300 GL column with an excitation wavelength of 330 nm and a monitoring wavelength of 420 nm.