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Substrate preferences of rAly5 and its truncated T336N protein. Degradation of saturated oligosaccharides (∼ 20 μg) by enzymes at their optimal temperatures for 12 h. E (−), without the enzyme (control group). HPLC analyses were performed using a Superdex peptide 10/300 GL column monitored at a wavelength of 235 nm. G6, hexasaccharide of guluronate; G5, pentasaccharide of guluronate; M5, pentasaccharide of mannuronate.
