FIG 3.

MLSA-based relationship tree of the Pseudomonas strains. Concatenated gyrB, recA, rpoB, and 16S rRNA genes were used to construct a relationship tree of the Pseudomonas antagonistic strains (P. fluorescens PA3G8 and PA4C2, P. putida PA14H7, and P. brassicacearum PA1G7 and PP1-210F) and several well-identified strains of the Pseudomonas genus. An Azotobacter strain concatenated sequence was used to root the phylogenetic tree. The sequences were aligned with ClustalW, and then a neighbor-joining tree was created. The accession numbers of the NCBI sequences are indicated in parentheses.